Development and multiplex genotyping of eighteen novel microsatellite markers for a threatened saltmarsh bird Epthianura albifrons (Meliphagidae)
Contents
Abstract
Using 454 pyrosequencing and multiplex
genotyping we developed 18 novel microsatellite loci in
Epthianura albifrons (Jardine & Selby, 1828), a small
passerine threatened by loss of coastal saltmarsh. We also
tested the utility of 21 previously-developed microsatellite
loci from two con-familial species, however these were
weakly polymorphic in E. albifrons. The 18 loci developed
for E. albifrons were polymorphic with a mean of 17.1
(±2.6SE) alleles per locus. The 18 new microsatellites will
be useful tools for measuring gene flow in the Endangered
population of E. albifrons.
