Refereed Article Development and multiplex genotyping of eighteen novel microsatellite markers for a threatened saltmarsh bird Epthianura albifrons (Meliphagidae)

Citation: King A. G., Georgina M. Cooke, Rebecca N. Johnson, Richard E. Major. 2012. Development and multiplex genotyping of eighteen novel microsatellite markers for a threatened saltmarsh bird Epthianura albifrons (Meliphagidae). Conservation Genetics Resources. 4. (4): 893-896. ISSN 1877-7252

DOI: 10.1007/s12686-012-9667-9

Abstract:

Using 454 pyrosequencing and multiplex
genotyping we developed 18 novel microsatellite loci in
Epthianura albifrons (Jardine & Selby, 1828), a small
passerine threatened by loss of coastal saltmarsh. We also
tested the utility of 21 previously-developed microsatellite
loci from two con-familial species, however these were
weakly polymorphic in E. albifrons. The 18 loci developed
for E. albifrons were polymorphic with a mean of 17.1
(±2.6SE) alleles per locus. The 18 new microsatellites will
be useful tools for measuring gene flow in the Endangered
population of E. albifrons.

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